Publicação: Influência de dois agentes antioxidantes na camada híbrida de restaurações sujeitas a irrigação endodôntica : caracterização morfológica com microscopia eletrónica de varrimento
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Objetivos: Avaliar a influência de dois agentes antioxidantes na camada híbrida sujeita a irrigação com hipoclorito de sódio através da Microscopia Eletrónica de Varrimento (MEV).
Materiais e Métodos: Foram selecionados 15 molares hígidos, extraídos há menos de 6 meses do Banco de Dentes da Clínica Dentária Egas Moniz. Todos os dentes foram seccionados removendo 2 a 3 mm abaixo da junção amelo-cimentária, e de seguida seccionados a 4 mm da superfície oclusal expondo a dentina. Sobre a dentina exposta foi simulada uma restauração com a resina composta GrandioSO (Voco, Alemanha). Foi
utilizado o sistema adesivo etch-and-rinse de 3 passos Optibond FL™ (Kerr, USA). Após a restauração dos dentes, foi simulado um acesso endodôntico em oclusal.
Posteriormente, os dentes foram divididos em três grupos: Grupo controlo, n=5, em que as amostras foram somente irrigadas com hipoclorito de sódio (NaOCl); Grupo 1, n=5, em que as amostras foram irrigadas com NaOCl e posteriormente irrigadas com o agente antioxidante sintético Ascorbato de Sódio a 10%; e Grupo 2, n=5, em que as amostras foram irrigadas com NaOCl e posteriormente irrigadas com o agente antioxidante natural Proantocianidina a 5%.
Todos os dentes foram cortados exatamente a meio, paralelamente ao longo eixo do dente, para serem analisados com Microscopia Eletrónica de Varrimento (MEV).
Os resultados foram interpretados com recurso a estatística descritiva.
Resultados: Verificaram-se diferenças entre o Grupo controlo que apresentava um gap significativo na interface adesiva, o Grupo 1 que quase não apresentava gap e o Grupo 2 que não apresentava qualquer gap.
Conclusão: O NaOCl afeta a interface adesiva de restaurações pré-endodônticas, pois parece haver um fenómeno de remineralização decorrente do uso de antioxidante, seguido de armazenamento em saliva.
Purpose: To evaluate the influence of two antioxidant agents on the hybrid layer undergone irrigation with sodium hypochlorite through Scanning Electron Microscopy (SEM). Materials and Methods: 15 human molars, sound, extracted less than 6 months ago, were selected from the Egas Moniz Human Teeth Bank. The teeth were sectioned 2-3 mm below the amelocemental joint, and then sectioned 4 mm from the occlusal surface, exposing dentin. A restoration with the resin composite GrandioSO (Voco, Germany) was simulated on the exposed dentin. The 3-step etch-and-rinse adhesive system Optibond FL™ (Kerr, USA) was used. After the restoration of the teeth, an endodontic access was made in the occlusal surface. The teeth were then divided into three different groups: Control group, n=5, in which the samples were only irrigated with sodium hypochlorite; Group 1, n=5, in which the samples were irrigated with sodium hypochlorite and subsequently irrigated with the synthetic antioxidant agent Sodium Ascorbate 10%; and Group 2, n=5, in which the samples were irrigated with sodium hypochlorite and subsequently irrigated with the natural antioxidant agent Proanthocyanidin 5%. All the teeth were cut vertically, exactly in half, to be analyzed with Scanning Electron Microscopy (SEM). The results were submitted to a descriptive statistical analysis. Results: This study resulted in differences between the Control group, which presented a significant gap in the adhesive interface, Group 1, which presented nearly no gap and Group 2, which didn’t present any gap. Conclusion: NaOCl affects the adhesive interface of pre-endodontic restorations, for there appears to be a phenomenon of remineralization due to the use of the antioxidant agent, followed by storage in saliva.
Purpose: To evaluate the influence of two antioxidant agents on the hybrid layer undergone irrigation with sodium hypochlorite through Scanning Electron Microscopy (SEM). Materials and Methods: 15 human molars, sound, extracted less than 6 months ago, were selected from the Egas Moniz Human Teeth Bank. The teeth were sectioned 2-3 mm below the amelocemental joint, and then sectioned 4 mm from the occlusal surface, exposing dentin. A restoration with the resin composite GrandioSO (Voco, Germany) was simulated on the exposed dentin. The 3-step etch-and-rinse adhesive system Optibond FL™ (Kerr, USA) was used. After the restoration of the teeth, an endodontic access was made in the occlusal surface. The teeth were then divided into three different groups: Control group, n=5, in which the samples were only irrigated with sodium hypochlorite; Group 1, n=5, in which the samples were irrigated with sodium hypochlorite and subsequently irrigated with the synthetic antioxidant agent Sodium Ascorbate 10%; and Group 2, n=5, in which the samples were irrigated with sodium hypochlorite and subsequently irrigated with the natural antioxidant agent Proanthocyanidin 5%. All the teeth were cut vertically, exactly in half, to be analyzed with Scanning Electron Microscopy (SEM). The results were submitted to a descriptive statistical analysis. Results: This study resulted in differences between the Control group, which presented a significant gap in the adhesive interface, Group 1, which presented nearly no gap and Group 2, which didn’t present any gap. Conclusion: NaOCl affects the adhesive interface of pre-endodontic restorations, for there appears to be a phenomenon of remineralization due to the use of the antioxidant agent, followed by storage in saliva.
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Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
